杨崇林


教授/ clyang@ynu.edu.cn/细胞器稳态调控相关机制研究


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杨崇林

1998年于北京大学获博士学位。1998-1999年为意大利International School for Advanced Studies分子神经生物学实验室访问学者。1999-2005年先后在University of Maryland School of MedicineUniversity of Colorado, Boulder从事博士后研究。2005-2012年任中国科学院遗传与发育生物学研究所研究员。2006年入选中国科学院百人计划,终期获评为优秀2010年获国家杰出青年科学基金资助。2012年被评为全国优秀科技工作者2017年入选国家百千万人才工程,获有突出贡献中青年专家称号。2021年入选云南省“科技领军人才”。

一、研究方向:细胞器稳态调控相关机制研究

以秀丽线虫、培养细胞、小鼠等为模式,运用遗传、生化以及细胞生物学等研究方法,探索重要细胞器(线粒体、溶酶体)的稳态调控机制及其与遗传代谢性疾病之间的关系。


1.线粒体稳态与代谢

以秀丽线虫为模式,通过非偏好性的突变体筛选及基因克隆,发现调控线粒体结构、功能、融合-分裂等线粒体稳态维持的重要基因,从遗传互作、基因表达、蛋白定位和功能、线粒体结构功能、线粒体代谢等多角度开展研究,揭示线粒体稳态、代谢、及线粒体与其他细胞器互作对细胞稳态和功能的影响,揭示线粒体在遗传代谢性疾病中的作用。

(C. elegansmutants with abnormal mitochondria)



(Defective lysine catabolism causes mitochondrial damage)

(Images and quantification of Mito-CMXRos staining in adult animals of N2 and slc-30A9 mutants)


(A pair of zinc transporters controls mitochondrial zinc levels)



2.溶酶体稳态调控机制

运用遗传学、细胞生物学、化学生物学等方法,研究溶酶体发生、溶酶体运输、溶酶体降解的调控机制,探索溶酶体稳态维持在组织器官发育中的重要作用。筛选促进溶酶体发生的小分子化合物,阐明其作用机制并研究它们在神经退行性疾病中的作用。

(The amino acid transporter SLC-36.1 is required for lysosome reformation from phagolysosome during apoptotic cell corpse clearance)

(Time-lapse monitoring of early-to-late endosome conversion in HeLa cells)



(Lysosomes receive proteins and cargos from multiple pathways)

二、组员

工作人员:

尹秋媛博士,副研究员

张洁 博士,助理研究员

李婧琳 博士,助理研究员

刘楠 博士,助理研究员

段梅 博士,助理研究员

王鑫 硕士,研究实习员

李美娇 硕士,研究实习员

杨利梅 硕士,实验室管理员


在读研究生:

博士研究生:马腾飞、白艳、田飞、赵姗、杨扬、单倩

硕士研究生:秦子臻、孔前进、张宗豪、林登迅、石函冉、侯成儒、陈冠宇

三、发表文章(2006-present):

1. Junxiang Zhou, Mei Duan, Xin Wang, Fengxia Zhang, Hejiang Zhou, Tengfei Ma, Qiuyuan Yin, Jie Zhang, Fei Tian, Guodong Wang, andChonglin Yang. (2022). A feedback loop engaging propionate catabolism intermediates controls mitochondrial morphology.Nature Cell Biology24(4): 526-537.

2. Tengfei Ma, Liyuan Zhao, Jie Zhang, Ruofeng Tang, Xin Wang, Nan Liu, Qian Zhang, Fengyang Wang, Meijiao Li, Qian Shan, Yang Yang, Qiuyuan Yin, Limei Yang, Qiwen Gan, andChonglin Yang. (2021). A pair of transporters controls mitochondrial Zn2+levels to maintain mitochondrial homeostasis.Protein Celldoi: 10.1007/s13238-021-00881-4.

3.Chonglin Yang, and Xiaochen Wang. (2021). Lysosome biogenesis: Regulation and functions.Journal of Cell Biology220(6): e202102001.

4. Ruxiao Xing, Hejiang Zhou, Youli Jian, Lingling Li, Min Wang, Nan Liu, Qiuyuan Yin, Ziqi Liang, Weixiang Guo andChonglin Yang.(2021). The Rab7 effector WDR91 promotes autophagy-lysosome degradation in neurons by regulating lysosome fusion.Journal of Cell Biology220 (8): e202007061.

5. Yuan Li, Xin Wang, Meijiao Li,Chonglin Yang, Xiaochen Wang. (2021). M05B5.4 (lysosomal phospholipase A2) promotes disintegration of autophagic vesicles to maintainC. elegansdevelopment.Autophagydoi: 10.1080/15548627.2021.1943178.

6. Qiuyuan Yin, Youli Jian, Meng Xu, Xiahe Huang, Niya Wang, Zhifang Liu, Qian Li, Jinglin Li, Hejiang Zhou, Lin Xu, Yingchun Wang, andChonglin Yang. (2020). CDK4/6 regulate lysosome biogenesis through TFEB/TFE3.Journal of Cell Biology219(8): e201911036.

(JCB 2020 Editor’s Pick)

7. Ruofeng Tang, Xin Wang, Junxiang Zhou, Fengxia Zhang, Shan Zhao, Qiwen Gan, Liyuan Zhao, Fengyang Wang, Qian Zhang, Jie Zhang, Guodong Wang,Chonglin Yang. (2020). Defective arginine metabolism impairs mitochondrial homeostasis inCaenorhabditis elegans.Journal of Genetics and Genomics47: 145-156.

8.Rui Miao,Meijiao Li,Qianqian Zhang,Chonglin Yang,Xiaochen Wang. (2020). An ECM-to-nucleus signaling pathway activates lysosomes forC. eleganslarval development.Developmental Cell52(1): 21-37.(As a cover article)

9. Qiwen Gan, Xin Wang, Qian Zhang, Qiuyuan Yin, Youli Jian, Yubing Liu, Nan Xuan, Jinglin Li, Junxiang Zhou, Kai Liu, Yudong Jing, Xiaochen Wang, andChonglin Yang. (2019). The amino acid transporter SLC-36.1 cooperates with PtdIns3P 5-kinase to control phagocytic lysosome reformation.Journal of Cell Biology218 (8): 2619-2637.(As a cover article).

10.Junxiang Zhou, Xin Wang, Min Wang, Yuwei Chang, Fengxia Zhang, Zhaonan Ban, Ruofeng Tang, Qiwen Gan, Shaohuan Wu, Ye Guo, Qian Zhang, Fengyang Wang, Liyuan Zhao, Yudong Jing, Wenfeng Qian, Guodong Wang, Weixiang Guo, andChonglin Yang. (2019). The lysine catabolite saccharopine impairs development by disrupting mitochondrial homeostasis.Journal of Cell Biology218(2): 580-597.

(Spotlight:Saccharopine, a lysine degradation intermediate, is a mitochondrial toxin. João Leandro and Sander M. Houten.Journal of Cell Biology218(2): 391-392).

11. Yang Li, Yu Zhang, Qiwen Gan, Meng Xu, Xiao Ding, Guihua Tang, Jingjing Liang, Kai Liu, Xuezhao Liu, Xin Wang, Lingli Guo, Zhiyang Gao, Xiaojiang Hao,Chonglin Yang. (2018).C. elegans-based screen identifies lysosome-damaging alkaloids that induce STAT3-dependent lysosomal cell death.Protein Cell9(12): 1013–1026.

12. Caitlin Morris, Olivia K. Foster, Simran Handa, Kimberly Peloza, Laura Voss, Hannah Somhegyi, Youli Jian, My Van Vo, Marie Harp, Fiona M. Rambo,Chonglin Yang, Greg J. Hermann. (2018). Function and regulation of theCaenorhabditis elegansRab32 family member GLO-1 in lysosome-related organelle biogenesis.PLoS Genetics14(11): e1007772.

13. Xinli Ma, Kai Liu, Jian Li, Huanhuan Li, Jun Li, Yingfang Liu,Chonglin Yangand Huanhuan Liang.(2018). A non-canonical GTPase interaction enables ORP1L-Rab7-RILP complex formation and late endosome positioning.Journal of Biological Chemistry293(36): 14155-14164.

14.Kai Liu, Ruxiao Xing, Youli Jian, Zhiyang Gao, Xinli Ma, Xiaojuan Sun, Yang Li, Meng Xu, Xin Wang, Yudong Jing, Weixiang Guo andChonglin Yang. (2017). WDR91 is a Rab7 effector required for neuronal development.Journal of Cell Biology216(10): 3307-3321.

(Spotlight:A new Rab7 effector controls phosphoinositide conversion in endosome maturation. Casanova J. and Winckler B.Journal of Cell Biology216(10): 2995-2997).

15. Jianhua Yin, Yaling Huang, Pengfei Guo, Siqi Hu, Sawako Yoshina, Nan Xuan, Qiwen Gan, Shohei Mitani,Chonglin Yang, Xiaochen Wang. (2017). GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 inC. elegans.Journal of Cell Biology216(6): 1775-1794.

16. Xuezhao Liu, Yang Li, Xin Wang, Ruxiao Xing, Kai Liu, Qiwen Gan, Changyong Tang, Zhiyang Gao, Youli Jian, Shouqing Luo, Weixiang Guo, andChonglin Yang. (2017). The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.Journal of Cell Biology216(5): 1301-1320.

(JCB 2018 Special Collection on Mitochondria and Autophagy).

17.Chonglin Yang, Xiaochen Wang. (2017). Cell Biology in China: Focusing on the Lysosome.Traffic18(6): 348-357.

18. Shiya Cheng, Kai Liu,Chonglin Yang, Xiaochen Wang. (2017). Dissecting Phagocytic Removal of Apoptotic Cells inCaenorhabditis elegans. InMethods in Molecular Biology:Phagocytosis and Phagosomes(Eds. Roberto Botelho) Springer New York 1519: 265-284.

19. Yuan Li, Baohui Chen, Wei Zou, Xin Wang, Yanwei Wu, Dongfeng Zhao, Yanan Sun, Yubing Liu, Lianwan Chen, Long Miao,Chonglin Yang, Xiaochen Wang. (2016). The lysosomal membrane protein SCAV-3 maintains lysosome integrity and adult longevity.Journal of Cell Biology215(2): 167-185.

20. Yang Li, Meng Xu, Xiao Ding, Chen Yan, Zhiqin Song, Lianwan Chen, Xiahe Huang, XinWang, Youli Jian, Guihua Tang, Changyong Tang, Yingtong Di, Shuzhen Mu, Xuezhao Liu, Kai Liu, Ting Li,Yingchun Wang, Long Miao, Weixiang Guo, Xiaojiang Hao andChonglin Yang. (2016). Protein kinase C controls lysosome biogenesis independently of mTORC1.Nature Cell Biology18: 1065-1077.

(News and Views:Turn up the lysosome. Paul Saftig, Albert Haas.Nature Cell Biology18: 1025-1027).

21. Xiaochen Wang,Chonglin Yang. (2016). Programmed cell death and clearance of cell corpses inCaenorhabditis elegans.Cellular and Molecular Life Sciences73: 2221-2236.

22.Kai Liu, Youli Jian, Xiaojuan Sun, Chengkui Yang, Zhiyang Gao, Zhili Zhang, Xuezhao Liu, Yang Li, Jing Xu, Yudong Jing, Shohei Mitani, Sudan He,Chonglin Yang. (2016). Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion.Journal of Cell Biology212(2): 181-198.

(In Focus:Making sure late endosomes are on time. Mitch Leslie.Journal of Cell Biology212: 127).

23. Meng Xu, Yubing Liu, Liyuan Zhao, Qiwen Gan, Xiaochen Wang, andChonglin Yang.(2014). The lysosomal cathepsin protease CPL-1 plays a leading role in phagosomal degradation of apoptotic cells inCaenorhabditis elegans.Molecular Biology of the Cell25: 2071-2083.

24. Didi Chen, Youli Jian, Xuezhao Liu, Yuanya Zhang, Jingjing Liang, Xiaying Qi, Hongwei Du, Wei Zou, Lianwan Chen, Yongping Chai, Guangshuo Ou, Long Miao, Yingchun Wang,Chonglin Yang. (2013). Clathrin and AP2 are required for phagocytic receptor-mediated apoptotic cell clearance inCaenorhabditis elegans.PLoS Genetics9(5): e1003517.

25. Jing Xu, Xiaojuan Sun, Yudong Jing, Mo Wang, Kai Liu, Youli Jian, Mei Yang, Zhukuan Cheng,Chonglin Yang. (2012). MRG-1 is required for genomic integrity inCaenorhabditis elegansgerm cells.Cell Research22, 886-902.

26. Didi Chen, Hui Xiao, Kai Zhang, Bin Wang,Zhiyang Gao, Youli Jian, Xiaying Qi, Jianwei Sun, Long Miao,Chonglin Yang. (2010). Retromer is required for apoptotic cell clearance by phagocytic receptor recycling.Science327(5970): 1261-1264.

(Editor's Choice:Corpse-sorting machinery. S. M. Hurtley.Science Signaling2010, 3, ec73;Highlighted by Nature China).

27. Xiao Yang andChonglin Yang. (2010). An everlasting role of animal models in understanding human disease.Journal of Genetics and Genomics37(9): 557.

28. Youli Jian, Zhiyang Gao, Jianwei Sun, Qinfang Shen, Feng Feng, Yudong Jing andChonglin Yang. (2009). RNA aptamers interfering with nucleophosmin oligomerization induce apoptosis in cancer cells.Oncogene28(47): 4201-4211.

29. Qinfang Shen, Fengsong Qin, Zhiyang Gao, Jie Cui, Hui Xiao, Zhiheng Xu, andChonglin Yang. (2009). Adenine Nucleotide Translocator Cooperates with Core Cell Death Machinery to Promote Apoptosis inCaenorhabditis elegans.Molecular and Cellular Biology29(14): 3881–3893.

30. Mei Yang, Jianwei Sun, Xiaojuan Sun, Qinfang Shen, Zhiyang Gao,Chonglin Yang. (2009).Caenorhabditis elegansProtein Arginine Methyltransferase PRMT-5 Negatively Regulates DNA Damage-Induced Apoptosis.PLoS Genetics5(6): e1000514.

31. Hui Xiao, Didi Chen, Zhou Fang, Xiaojuan Sun, Jing Xu, Song Song, Jiajia Liu andChonglin Yang. (2009). Lysosome biogenesis mediated byvps-18affects apoptotic cell degradation inCaenorhabditis elegans.Molecular Biology of the Cell20(1): 21-32.

32.Chonglin Yang, Myoung Sook Kim, Devulapalli Chakravarty, Fred E. Indig and France Carrier. (2009). Nucleolin Binds to the Proliferating Cell Nuclear Antigen and Inhibits Nucleotide Excision Repair.Molecular and Cellular Pharmacology1(3):130-137.

33. Xiaochen Wang, Jin Wang, Keiko Gengyo-Ando, Lichuan Gu, Chun-Ling Sun,Chonglin Yang, Yong Shi, Tetsuo Kobayashi, Yigong Shi, Shohei Mitani, Xiaosong Xie and Ding Xue. (2007).C. elegansmitochondrial factor WAH-1 promotes phosphatidylserine externalization in apoptotic cells through phospholipid scramblase SCRM-1.Nature Cell Biology9(5): 541-549.

34.Chonglin Yang, Nieng Yan, Jay Parish, Xiaochen Wang, Yigong Shi and Ding Xue. (2006). RNA aptamers targeting the cell death inhibitor CED-9 induce cell killing inC. elegans.Journal of Biological Chemistry281(14): 9137-9144.

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